更新時(shí)間:2024-06-11
我司Mouse podocyte(小鼠足細(xì)胞)的細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無(wú)機(jī)離子及其他一些入核酸降解物、激素等。保證運(yùn)輸中的正常生長(zhǎng),關(guān)于其價(jià)格、報(bào)價(jià)、貨期,已打造成抗體在華東地區(qū)Z大交易平臺(tái)之一,歡迎您的咨詢!
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Mouse podocyte(小鼠足細(xì)胞)對(duì)于每種細(xì)胞因子應(yīng)仔細(xì)閱讀說(shuō)明書,注意每批的細(xì)節(jié)。在使用細(xì)胞因子前,瞬時(shí)離心試劑瓶,盡可能多地收回其中的細(xì)胞因子。
Mouse podocyte(小鼠足細(xì)胞)來(lái)源可靠(源于ATCC、ECACC、DSMZ、JCRB等),活力>95%,貼壁性好。我們從試劑、包被器皿、凍存原代細(xì)胞、新鮮原代細(xì)胞、原代細(xì)胞的分子學(xué)實(shí)驗(yàn)等提供全程服務(wù)。我司擁有專業(yè)的實(shí)驗(yàn)室,可無(wú)菌操作并提供相關(guān)科研項(xiàng)目解決方案以及實(shí)驗(yàn)服務(wù)。
服務(wù)流程:
預(yù)約登記→辦理相關(guān)手續(xù)→復(fù)蘇細(xì)胞→細(xì)胞質(zhì)量檢查→發(fā)送細(xì)胞(或自己來(lái)取細(xì)胞)→細(xì)胞售后技術(shù)咨詢答疑。
養(yǎng)方法如下:
1、從手術(shù)室無(wú)菌取腦髓灰質(zhì)或白質(zhì)后,仔細(xì)剝除腦膜、血管和纖維成分,置Hanks液中漂洗一、二次。
2、置于30—50倍體積的Hanks液中,此時(shí)腦組織比較柔軟,反復(fù)吹打即制成細(xì)胞懸液。
3、把懸液注入離心管室溫中直立5—10分鐘后,細(xì)胞或細(xì)胞團(tuán)快自然下沉,脂肪等雜物易漂浮,可吸除上層、反復(fù)二、三次,即可排除脂肪成分和其它碎塊并獲較多細(xì)胞成分。
4、在沉降物中加入適量培養(yǎng)液,通過(guò)紗網(wǎng)成紗布過(guò)濾,計(jì)數(shù)并調(diào)整細(xì)胞密度。
5、接入培養(yǎng)瓶或皿中,置5%CO2溫箱中培養(yǎng)。
適應(yīng)環(huán)境過(guò)程較長(zhǎng),接種后貼壁較慢。貼壁后短期內(nèi)也可能不見細(xì)胞分裂現(xiàn)象,然而一旦生長(zhǎng)后即能迅速進(jìn)入旺盛的增殖狀態(tài)。細(xì)胞傳代可以0.25%胰酶消化處理。
我司其他相關(guān)銷售產(chǎn)品:
Cs-(035)-007239 COC1/CDDP細(xì)胞株/細(xì)胞,卵巢癌順鉑耐藥株 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007240 CNE細(xì)胞株/細(xì)胞,鼻咽癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007241 CNE細(xì)胞株/細(xì)胞,鼻咽癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007242 CNE鼻咽癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007243 CNE2細(xì)胞株/細(xì)胞,鼻咽癌細(xì)胞株/細(xì)胞(低分化) ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007244 CNE-2Z細(xì)胞株/細(xì)胞,鼻咽癌母系細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007245 CNE1細(xì)胞株/細(xì)胞,鼻咽癌細(xì)胞株/細(xì)胞(高分化) ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007246 CNE-1細(xì)胞株/細(xì)胞,高分化鼻咽癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007247 CNE1-lmp\l細(xì)胞株/細(xì)胞,轉(zhuǎn)基因鼻咽癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007248 CNE(鼻咽癌細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007249 CMT93細(xì)胞株/細(xì)胞,結(jié)腸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007250 CL187/CCL187細(xì)胞株/細(xì)胞,大腸癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007251 Cl.Ly1+2-/9細(xì)胞株/細(xì)胞,T淋巴細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007252 CIK細(xì)胞株/細(xì)胞,草魚腎臟細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007253 CHSE細(xì)胞株/細(xì)胞,鮭魚胚胎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007254 CHRF細(xì)胞株/細(xì)胞,巨核細(xì)胞株/細(xì)胞白血病 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007255 CHRF(巨核細(xì)胞株/細(xì)胞白血?。?nbsp; ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007256 CHO細(xì)胞株/細(xì)胞,中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007257 CHO-pt細(xì)胞株/細(xì)胞,轉(zhuǎn)血小板基因倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007259 CHO-K1倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞亞株 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007260 CHOdhfr細(xì)胞株/細(xì)胞,二氫葉酸缺陷型中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007264 CHO/dhFr-(中國(guó)倉(cāng)鼠二氫葉酸還原酶缺陷細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007265 CHO(中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007275 CFSC-8B細(xì)胞株/細(xì)胞,肝星形細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007276 CFSC-3H & 5H細(xì)胞株/細(xì)胞,肝星形細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007281 CEM細(xì)胞株/細(xì)胞,白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007284 CCRF-CEM急性淋巴細(xì)胞株/細(xì)胞白血病T淋巴細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007287 CCD-1095Sk細(xì)胞株/細(xì)胞,皮膚細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-007291 CCC-HSF-1細(xì)胞株/細(xì)胞,皮膚成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-007292 CCC-HPF-1細(xì)胞株/細(xì)胞,胚肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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